Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation HOME Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation.
Effect of RGD functionalization and stiffness modulation of polyelectrolyte multilayer films on muscle cell differentiation. Download PDF. Although arginylglycylaspartic acid RGD substrates have been widely explored in tissue engineering, there have been no studies aimed at investigating the combined effects of RGD nanoscale presentation and matrix stiffness on myogenesis.
C2C12 myoblasts were used as cellular models. Published by Elsevier Ltd. All rights reserved. Introduction Regenerative medicine and tissue engineering make use of injected cells or of biomaterials to support cell attachment and to provide them with the appropriate cues to guide their differentiation. Skeletal muscle tissue engineering holds the promise of the replacement of muscle damaged by injury following surgery or due to trauma and for the treatment of muscle diseases, such as muscular dystrophy or paralysis.
Adult skeletal muscle progenitor cells are considered a powerful source for the generation of several tissues, especially skeletal muscle, [1] but also smooth muscle [2], bone [3,4] and fat tissue [5,6]. E-mail address: [email protected] C. Proliferation and differentiation of skeletal myoblasts are mutually exclusive events, which are governed by the up-regulation of transcriptional activators [7].
A major limitation to the clinical application of muscle progenitors is rapid loss of their muscle stem cell properties once they are removed from their in vivo environment [8].
