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Capillary electrophoresis with fluorescence detection has been investigated for simple, sensitive, and selective analysis of morphine and 6-acetylmorphine 6-AM in human urine. The method is selective in the presence of codeine. When fluorescence detection was used with an excitation wavelength of nm and an emission cutoff filter of nm, good linearity was obtained in the range of 50— ng mL —1 with limits of detection and quantification below 1.
The method was applied to human urine and validated by comparison with previously established capillary electrophoretic methods. Accuracy, repeatability, and intermediate precision of results were comparable. The method is suitable for application in forensic cases for initial screening, and in clinical analysis to prevent overdose-induced toxicity.
Stjernsward Cancer Surv. Glass S. Ingalls C. Schilling C. Hoppel J. Goldberger W. Darwin T. Grant A. Allen Y. Caplan E. Cone J. Dybowski T. Gough J. Kerrigan B. Goldberger B. Swinney D. Bornhop Electrophoresis 21 Breadmore P. Haddad Electrophoresis 22 Fukushima N. Usui T. Santa K.
Imai J. Ichinose G. Schwedt F. Schnepel K. Adachi I. Aderjan S. Hofmann G. Schmitt G. Skopp J. Crump I. McIntyre O. Drummer J. Paez P. Rada S. Tucci N. Rodriguez L. Hernandez J. A Kanazawa T. Nagatsuka M. Miyazaki Y. Matsushima J. Cohen F. Armstrong H. Campbell J. Kataoka J. Underberg J. Waterval Electrophoresis 23 Li J. Miyano T. Toyo'oka K. Imai Anal. Acta Liu Y. Hsieh D. Wiesler M. Novotny Anal. Rama Rao S. Tandon Anal. Groth G. Wallerberg Acta Chem. Alnajjar J. Butcher B.
McCord Electrophoresis 25 Mofwa K. Chain Y. Hashimoto Legal Med. Bosch A. Sanchez F. Rojas C. Ojeda J. Biomed Anal. Alnajjar B. McCord J. Sign in Sign up. Advanced Search Help. Acta Chromatographica. Capillary electrophoresis with fluorescence detection for sensitive analysis of morphine and 6-acetylmorphine in human urine.