Official websites use. Share sensitive information only on official, secure websites. Corresponding author. Phone: 33 4 91 32 45 Fax: 33 4 91 32 46 E-mail: Jean-Marie. PAGES medecine. Both strains showed a porin pattern different from that of a susceptible strain, with a drastic reduction in the amount of the major porin but with an apparently conserved normal structure size and immunogenicity , together with overproduction of two known outer membrane proteins, OmpX and LamB.
In addition, the full-length O-polysaccharide phenotype was replaced by a semirough Ra phenotype. Moreover, in one isolate the intracellular accumulation of chloramphenicol was increased in the presence of the energy uncoupler carbonyl cyanide m -chlorophenylhydrazone, suggesting an energy-dependent efflux of chloramphenicol in this strain. The resistance strategies used by these isolates appear to be similar to that induced by stress in Escherichia coli cells.
Bacteria have developed various regulatory systems which coordinate their adaptive responses with the different environmental stresses to which they are exposed. Under these conditions, the expression of several genes appeared to be significantly activated or down-regulated. These modulations of gene expression alter the sensitivities of the bacteria to a broad range of antibiotics 1 , 2. Enterobacter aerogenes is one of the more frequently described gram-negative bacteria responsible for nosocomial respiratory tract infections 5 , Moreover, E.
Drug efflux can be coincident with a drastic reduction in drug uptake due to the loss of porin content 16 , 22 , 23 , Two E. The aim of this work was to examine some factors that may contribute to the resistance to antimicrobial agents in these two E.
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Susceptibilities to antibiotics were determined by a twofold standard broth microdilution method Drug interaction was performed by the same dilution method described above. Outer membrane proteins were prepared from cultures in the exponential growth phase in Mueller-Hinton broth by ultrasonic treatment, followed by ultracentrifugation and differential solubilization of the cytoplasmic material with sodium lauryl sarcosinate 0.
