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No use, distribution or reproduction is permitted which does not comply with these terms. Virus-like particles VLPs have gained interest over the last years as recombinant vaccine formats, as they generate a strong immune response and present storage and distribution advantages compared to conventional vaccines. Therefore, VLPs are being regarded as potential vaccine candidates for several diseases.
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One requirement for their further clinical testing is the development of scalable processes and production platforms for cell-based viral particles. In this work, the extended gene expression EGE method, which consists in consecutive media replacements combined with cell retransfections, was successfully optimized and transferred to a bioreactor operating in perfusion.
A process optimization using design of experiments DoE was carried out to obtain optimal values for the time of retransfection, the cell specific perfusion rate CSPR and transfected DNA concentration, improving Moreover, it was successfully implemented at 1.
VLP interaction with the ATF hollow fibers was studied via confocal microscopy, field emission scanning electron microscopy, and nanoparticle tracking analysis to design a bioprocess capable of separating unassembled Gag monomers and concentrate VLPs in one step. Since the human immunodeficiency virus 1 HIV-1 was first reported in , Due to the high impact and importance of this disease, many efforts have been devoted to understand and characterize the mechanisms taking place in HIV infection and replication von Schwedler et al.
